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Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), <t>mek-2(ku114)</t> , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in
Mek 2 Rnai Vector, supplied by Source BioScience plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in

Journal: Nature cell biology

Article Title: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

doi: 10.1038/ncb3470

Figure Lengend Snippet: Insulin-like signalling to the maternal germline modifies progeny response to osmotic stress by regulating the RAS-ERK-like pathway. (a) Percent of wild-type and lin-45(n2018), mek-2(ku114) , mpk-1(n5639) and lin-45(n2018); gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of >100 animals (b) Percent of wild-type and lin-45(n2018) cross progeny failing to arrest development at 500 mM NaCl after 48 hrs. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of >20 animals. (c) Representative germlines dissected from wild-type animals exposed to either 50 mM NaCl or 300 mM NaCl and stained for DNA (DAPI, white) and diphosphorylated MPK-1 (dpMPK-1) (red) Each condition was replicated 16 times. Scale bar 50 μm (d) Relative expression of gpdh-2 mRNA in wild-type and lin-45(n2018) embryos measured by qRT-PCR and normalized to the expression of the histone his-24. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos (e) Glycerol-to-glucose ratio in wild-type and lin-45(n2018) mutant embryos. Error bars, s.d. n = 3 experiments from pellets of >1000 embryos. The quantified results are presented as mean ± s.d. using ANOVA (a, b) and two-tailed t-test (d, e). * P < 0.05, ** P < 0.01, *** P < 0.001 were considered significant. n.s., not significant. See Statistics Source Data in

Article Snippet: Wild-type embryos fed Escherichia coli HT115 containing either the empty vector L4440 or a mek-2 RNAi vector (Ahringer library - Source Biosciences) were grown at 20°C for 72 hrs.

Techniques: Staining, Expressing, Quantitative RT-PCR, Mutagenesis, Two Tailed Test